Short Time Exposure Test

OECD 491

The Statens Seruminstitut rabbit cornea cell-based STE test method (OECD 491) is an in vitro cytotoxicity test. Cell viability is measured after a 5-minute exposure at both 5% and 0.05% test material concentrations to classify GHS NC and GHS category 1 chemicals. No predictions can be made for GHS category 2B and 2A chemicals. While the cell line is not differentiated, this is balanced by the rapid exposure of cells to the test substance followed by an evaluation of viability using the MTT assay. For the detection of GHS NC chemicals, the STE test method has an accuracy of 85%, a FN rate of 12%, and a FP rate of 19% (OECD 491). Test chemicals with vapor pressures higher than 6 kPa that fail to dissolve or form a suspension in mineral oil are outside of the application domain of the test method.

Lebrun Labs LLC follows the procedure described in OECD 491.

Additional Reading:
Same-chemical comparison of nonanimal eye irritation test methods: Bovine Corneal Opacity and Permeability, EpiOcular™, Isolated Chicken Eye, Ocular Irritection®, OptiSafe™, and Short Time Exposure

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