Recently, we compared OptiSafe with the other nonanimal eye irritation tests: Same chemical comparison of nonanimal eye irritation test methods: Bovine corneal opacity and permeability, EpiOcular™, isolated chicken eye, ocular Irritection®, OptiSafe™, and short time exposure
OptiSafe was the only nonanimal eye irritation test method to have no false negatives. Therefore, this test method is a good choice for initial product and formulation screening. Negatives can be accepted with a high confidence. Below is a summary of the comparison data:
Accuracy is the number of correctly predicted chemicals out of all chemicals tested.
As shown on the table above, OptiSafe has the highest comparative accuracy.
Sensitivity is the number of correctly predicted positive (irritant) chemicals.
As shown on the table above, OptiSafe has a very high sensitivity.
Specificity is the number of correctly predicted negative chemicals.
As shown on the table above, OptiSafe has a comparable specificity with other test methods with the exception of the STE method, which has a much lower sensitivity (see above table).
The false-negative rate is the number of true positives incorrectly predicted as negatives.
As shown on the table above, OptiSafe has the lowest false-negative rate (zero) of all the test methods evaluated.
The false-positive rate is the number of true negatives incorrectly predicted as positives.
OptiSafe has a comparable false-positive rate except for the comparison with the STE method. However, the STE method has a high false-negative rate (see above table).
Overall, OptiSafe has a comparable specificity and false-positive rate and a better accuracy and much better sensitivity (100%) and false-negative rates (0). Most remarkable is the finding that there were no OptiSafe false negatives. In addition to better accuracy and very high sensitivity, other features of the test include: does not use or harm animals, does not require aseptic technique, has a rapid turn-around-time, and is economical and shelf stable. OptiSafe is a good first choice for the initial eye safety evaluation of personal care products, makeup, and a wide range of consumer products and chemicals.