The OptiSafe test is a novel, shelf-stable, test tube-based test that can be used to discriminate ocular irritants/corrosives from nonirritants and does not use animal tissues or cells. The test method determines if an unknown substance is an ocular nonirritant by measuring and comparing chemical models for three variables: 1) damage to the corneal stroma (water-soluble molecules), 2) damage to phospholipid bilayers (water-insoluble molecules), and 3) the potential to induce pH extremes in a system (pH buffering system of the eye).
Lebrun Labs LLC has been developing and improving the in vitro depth of injury (IVD) ocular irritation test method to serve as an accurate, reliable way to test consumer products and chemicals for their irritancy potential. The purpose of this test method is to detect the full range of ocular irritants; including reversible damage. Although this test method is still in development, we welcome collaborations with interested parties. In addition, we provide customized “research-level” DoI testing services.