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RTA Colorimetric Kit : Microtiter Strip Format
Item#: RTA16X12
Our Price:
16 Strips For $192
80 Strips For $640

Main Features ╗

192 microtiter wells plus all reagents and rack with 96 well footprint. Microtiter wells coated with zeta-grip substrate. All reagents for colorimetric antigen assay except antigens, conjugate and serums. Complete colorimetric assay development kit.

The three-dimensional microarray substrate captures and protects proteins in a porous membrane. Proteins printed onto the array surface enter the membrane and maintain their integrity, providing increased sensitivity and assay consistency as well as high-binding capacity.

Standard protocol:

1. Dilute antigens in the spotting/printing buffer (provided).
2. After printing, allow to dry
Printed microtiter wells and slides are stable at room temperature for 3-12 months (depending on the antigen).
4. Add 200 Ál z-blocker (provided) to each microtiter 30 min (depending on assay).
5. Add a user-determined amount (or titrate) of sample or control (primary antibody) solution into individual wells and incubate with agitation for 30 minutes.
6. Appropriately discard the sample or control solution.
7. Wash by performing three quick rinses with wash buffer. In some cases, this wash step can be omitted.
8. Empty incubation dishes and appropriately dispose of wash solutions.
9. Add 10 ml of wash buffer to each incubation dish.
10. Add alkaline phosphatase-conjugated secondary antibody to each incubation dish
11. Incubate at room temperature with gentle agitation for 30 min.
12. Discard the solutions from the incubation dishes.
13. Wash by performing 3 quick rinses with wash buffer (provided).
14. Freshly prepare developer by adding 10 Ál NBT-BCIP/1 ml detection buffer (provided).
Add 10 ml developer to each chip or 100 Ál to each microtiter well. Incubate chips at room temperature for 12 minutes.
15. Discard developer and rinse with COLD tap water (holding under a faucet is okay). OPTIONAL: cover the chips with cold water for 2 minutes to stop further color development. Discard water.
16. Air-dry chips by positioning to allow excess water to run off. Signal will improve when dry.
17. Scan the slides and analyze data according to the Scanning and Data Analysis instructions.

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