The Hens Egg Test (HET) evaluates the potential for a test substance to induce toxicity in the chorioallantoic membrane (CAM) of an egg. Effects are measured by the onset of (1) hemorrhage, (2) coagulation, and (3) vessel lysis. These assessments are considered individually and are then used to derive a score. The score is then related to a prediction model to determine an eye irritancy classification. The HET-CAM test is used for a weight of evidence approach to determine possible eye irritancy of a substance being tested.
Lebrun Labs follows the ICCVAM-Recommended Test Method Protocol: Hen’s Egg Test – Chorioallantoic Membrane (HET-CAM) Test Method https://ntp.niehs.nih.gov/iccvam/docs/protocols/ivocular-hetcam.pdf