Skin Irritation Test (SIT)
The EpidermTM RhE test system uses human derived non-transformed keratinocytes as cell source to reconstruct an epidermal model used for skin irritation testing. Cell viability is measured by live cell metabolic conversion of the vital dye MTT, into a blue formazan salt that is quantitatively measured after extraction from tissues. Irritant chemicals are identified by their ability to decrease cell viability below defined levels below controls.
Lebrun Labs follows the procedure descrbed in OECD 439
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