Lebrun Labs LLC has developed the in vitro depth of injury (IVD) ocular irritation test method. The purpose of this test method is to detect the full range of ocular irritants, including those that are reversible.
 
This research is being funded by a National Institute of Environmental Health Sciences (NIEHS) phase II research grant:
 
Lebrun Labs LLC Recently Awarded a phase II SBIR Grant to Develop The IVD Eye Irritation Test
June 2020 
GRANT NUMBER: R44ES031881
GRANT TITLE: IVD EIT: A Nonanimal Kit and Service for the Detection of Reversible Ocular Irritants
Award Info: National Institute of Environmental Health Sciences (NIEHS) Small Business Innovative Research (SBIR) Grant. Research was supported by the NIEHS of the National Institutes of Health under Award Number R44ES031881. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health.

Journal Publication in Toxicology in Vitro
December 2019
An in vitro depth of injury prediction model for a histopathologic classification of EPA and GHS eye irritants
Stewart Lebrun, Yilu Xie, Sara Chavez, Roxanne Chan, James V. Jester

We developed an IVD prediction model to measure and classify the toxic effects of ocular irritants. This approach uses food-source, otherwise discarded eyes ("ex vivo"), that are exposed to potential ocular irritants. Following the subsequent rinsing of the eyes and 24 hours of organ culture, the DoI to the cornea is measured histopathologically using proprietary methods. The data are then quantified and related to a prediction model that results in an ocular irritancy classification, including the GHS 2A/2B reversible irritant classification.  
 

We welcome interested parties and stakeholders to partner and collaborate with us as well as use our IVD research-level testing services.  

Collaboration requests should be directed to:
Lebrun Labs LLC 
sales@lebrunlabs.com