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Easy WL-N Kit
Item#: W1004CP60B
Our Price:
32 tests For $349




Main Features 

An easy way to determine total aerobic counts and sterility. Use before bottling or any time you want to check total microbial levels; Cultivates yeast, mold, and bacteria encountered in brewing and industrial fermentation processes. Includes droppers, sterile beads, incubation bags, and WL Nutrient Agar Plates.

Intended Use:

WL medium is used for cultivating bacteria encountered in the wine, beer and industrial brewing industries.

WL medium was developed by Wallerstein laboratory in the 1950s for the identification of microbes encountered in brewing industries.

Note: WL Medium is a rich growth medium that supports a wide variety of bacteria. Everything that comes into contact with the medium besides the sample being tested should be sterile. All steps should be performed such that the chance of contamination is minimized.

Procedure:

Collect sample using supplied sterile bulb pipette. If sample is to be stored use a sterile collection tube.
Apply 1 drop (50uL) directly to agar using supplied dropper
Add 1 tube worth of supplied glass beads, cover plate.
With plate flat on the table, slide/shake plate back and forth about 10 times.
Remove top and invert plate over waste container to dispose of beads. Replace top.
Incubate plates at 25C30C in supplied self-sealing incubation bags.

Interpretation:
Total aerobic counts;
If single colonies develop, multiply by 20 to get colony forming units (CFU) per ml. If a haze develops over the entire plate and/or the plate turns yellow, your sample is TNTC (To Numerous To Count) and contains over 10,000 CFU/ml. If you get a TNTC result: Repeat the assay with serial 1:10 dilutions in sterile tubes using sterile buffer to determine exact cell concentration.

Presumptive identification: Specific growth characteristics and colony morphology may be indicative of specific microbes. See Edwards Illustrated guide to microbes in Beer and Wine
http://bookstore.ucdavis.edu/Display.cfm?itemId=2517

Precautions
Use sterile technique whenever plates are open.

Minimize the time that the top is off from the dish. Work in a draft free environment.

Avoid directly touching the agar medium.

Seal plates within supplied self-sealing bags to reduce plate drying.

Storage
Store refrigerated between 2-8C.
Shelf life: 3 months


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