EASY BAD MEDIUM
Item#: w110C60
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12 tests For $229
32 tests For $479



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Intended Use:

For the detection and cell counts of Dekkera/Brettanomyces and acid producing bacterium in juice or wine samples. Sample from the bottom or mix before taking sample. A few hours before you start, remove Parafilm from edges of each plate and allow plates to pre-warm and for water droplets to evaporate.
Procedure:
1. Apply 5 drops (200uL) in circular pattern directly to agar using supplied dropper
2.Add 1 tube worth of supplied glass beads, cover plate.
3. With plate flat on the table, slide/shake plate back in forth about 10 times.
4. Remove top and invert plate over waste container to dispose of beads.
5. Incubate plates at 25șC?0șC (room temperature is okay) in supplied self-sealing incubation bags.
6. Examine the plates after several days of incubation; look/smell for colour change and phenolic odor, after 4-12 days look for colonies. Record the number of colonies

Interpretation:
This media is differential for Brettanomyces and acid producing bacterium. Brettanomyces produces cheesy white colonies, a strong phenolic odour and if at high concentrations the medium colour will change from blue to green and then finally yellow. With extended incubations, Brett colonies will turn green. Acid producing bacteria may not produce distinct colonies or will produce flat colonies. If acid producing organisms are present, the medium will turn yellow, indicating that the pH has been changed from 5.5 to below 3.8.
Consider being positive if:

1. The agar colour changes from blue to yellow.
2. A phenolic medicinal or leathery odor is detected when opening the bag/plate.

If you get a positive result:

1. Filter, use SO2 or otherwise sanitize the wine.
2. Retest after remediation to make sure effective.