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Differential Brettanomyces Media 60mm Agar Plates
Item#: W1002P60
Our Price:
48 plates For $579


 

View powerpoint presentation on this product:  31_~Daniel Gomez article about Brett.pdf  



Main Features 

Enrichment formula allows for identification of Brettanomyces based on phenolioc odour and color change and quantification by colony-counting.

Intended Use
For the detection of Dekkera/Brettanomyces in juice or wine samples.

Brettanomyces tends to settle to the bottom of barrels/tanks and bottom samples should (or mixed wine samples) are recommended.

Procedure
1. Apply 50 uL of wine directly to plate using a sterile (or clean) pipette -- OR
Concentrate wine by filtering through a 0.45-m pore filtration membrane or centrifugation.
2. Spread wine sample -- OR apply filter directly to agar.
3. Examine after 4-14 days, recording color change and phenolic odor.

Interpretation

This media is selective and differential for Brettanomyces and is enriched to assure relatively fast growth of this slow-growing organism.
Consider being positive if:
1. Colony growth is detected between 4-14 days.Colony appearance is intially cheesy, whitish-yellow later turning green.
2. The agar colour changes from blue/green to light green (low cell number) or yellow (high cell number); can occur within 4-12 days.
3. A phenolic medicinal or leathery odor is detected when opening the bag/plate. Can occur in 48 hours or longer. Suggested: Compare odor to Brett sensory set (sold separately).

If single colonies develop, multiply by 20 to get colony forming units (CFU) per ml. If color change and development of phenolic odor is rapid and a haze develops over the entire plate, your sample is TNTC (To Numerous To Count) and contains over 10,000 CFU/ml. Brett phenolic metabolites taint the wine when Brett
If you get a TNTC result:

1. Immediately filter or otherwise sanitize the wine
2. Repeat the assay with serial 1:10 dilutions to determine exact cell concentration and confirm the extent of Brett contamination.
3.Retest after remediation to make sure effective.

Precautions

To avoid cross contamination of the winery environment, it is recommended that after the test is complete 10% bleach or 70% alcohol is applied to the plate and beads prior to disposal.

Minimize the time that the top is off from the dish. Work in a draft free environment.
Avoid directly touching the agar medium.


Storage Store refrigerated between 2-8C.
Shelf life: 4 months

Lebrun Labs LLC
5475 E. La Palma Ave. Ste 206
Anaheim, CA 92807
(949) 246-0339
http://lebrunlabs.com
sales@lebrunlabs.com


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