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RTA Colorimetric Kit: 96 well Plate format
Item#: RTA 2001
Our Price:
2 Plates For $198
10 Plates For $750




Main Features »

Complete colorimetric antigen assay development kit.

Reagent Materials provided:
96-well plate microtiter:
· z-Grip 96-well plate microtiter plates
· Blocking solution
· Wash buffer
· NBT/BCIP
· Detection buffer solution
· Positive control

Important Note:
This assay is not compatible with any detergent, including Tween 20 and SDS.


INTRODUCTION
This kit is intended for protein/antigen assays. Proteins are applied to the z-Grip surface manually or by a robotic spotting device.


The zeta-Grip RTA colorimetric array system has been developed for printing multiple antigens and detecting levels of antigen specific antibodies (e.g., antibodies in human serum). For sandwich arrays, we recommend the zeta-Grip RTA avidin surface (direct inquiries to: sales@lebrunlabs.com).


PROCEDURE
Sample preparation & Spotting
1. Dilute antigens in printing buffer (supplied).
2. After printing, allow zeta-Grip chips to dry
3. Printed microtiter wells have a 6 months–2 year shelf life (depending on the antigen).

Processing
4. Add 300 uL Blocking Solution to each microtiter well and gently agitate at room temperature or 37 °C for 30 min.
5. Add primary antibody solution and incubate with gentle agitation for 30 minutes. at room temperature.
6. Wash by adding 300 µL Wash Buffer, incubating 1–2 minutes with agitation and dumping wash solution as above. Repeat 2–3 times.
7. Add 200 µL of wash Buffer with pre-diluted AP-conjugated secondary antibody.
9. Incubate at room temperature with gentle agitation for 30 min.
10. Wash by performing three quick rinses with Wash Buffer.

Detection
11. Freshly prepare developer by adding 10 uL NBT-BCIP to each 1 mL of Detection Buffer.
12. Add 200 uL to each microtiter well. Incubate chips at room temperature for 12 minutes.

13. Discard the developer and rinse with tap water (holding under the faucet is okay). Optional: Cover the chips with cold water for 2 minutes to stop further color development. Discard water.
14. Air-dry by allowing excess water to run off. Signal will improve when dry. Optional: Use forced air for rapid drying.
15. After drying, scan the slides and analyze data according to the Scanning and Data Analysis Instructions.


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